Disposable Microscope Objective Lenses for Fluorescence Correlation Spectroscopy Using Latex Microspheres

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• 作者：Je&#769 ; rô ; me Wenger ; Davy Ge&#769 ; rard ; Heykel Aouani ; Herve&#769 ; Rigneault
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：September 1, 2008
• 年：2008
• 卷：80
• 期：17
• 页码：6800-6804
• 全文大小：254K
• 年卷期：v.80,no.17(September 1, 2008)
• ISSN：1520-6882

文摘

We explore the combination of a latex microsphere with a low NA lens to form a high performance optical system, and enable the detection of single molecules by fluorescence correlation spectroscopy (FCS). Viable FCS experiments at concentrations 1−1000 nM with different objectives costing less than $40 are demonstrated. This offers a simple and low-cost alternative to the conventional complex microscope objectives.