Structure-Physicochemical Function Relationships of Soybean -Conglycinin Heterotrimers
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- 作者：Nobuyuki Maruyama ; Mohamad Ramlan Mohamed Salleh ; Koji Takahashi ; Kazuhiro Yagasaki ; Hideyuki Goto ; Naho Hontani ; Shuko Nakagawa ; and Shigeru Utsumi
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2002
- 出版时间：July 17, 2002
- 年：2002
- 卷：50
- 期：15
- 页码：4323 - 4326
- 全文大小：61K
- 年卷期：v.50,no.15(July 17, 2002)
- ISSN：1520-5118
文摘
We purified four single molecular species of 
-conglycinin heterotrimers consisting of the 
and subunits or the ' and subunits from mutant soybean cultivars lacking the or ' subunit,respectively, and examined their structural features and physicochemical functions. The extent ofthe hydrophobicities of the heterotrimers was related to the number of the or ' subunit. The thermalstabilities of the heterotrimers were mainly conferred by the subunit which had lower thermal stability.Solubilities at low ionic strength (
= 0.08) of the heterotrimers containing the or ' subunit werevery similar to those of the and ' homotrimers, respectively. Emulsifying abilities and heat-inducedassociations of the heterotrimers containing one subunit were similar to those of the or 'homotrimer, whereas those of the heterotrimers containing two subunits were similar to those ofthe homotrimer.Keywords: -Conglycinin; structure-physicochemical function relationship; solubility; thermal stability;soybean
-conglycinin heterotrimers consisting of the and subunits or the ' and subunits from mutant soybean cultivars lacking the or ' subunit,respectively, and examined their structural features and physicochemical functions. The extent ofthe hydrophobicities of the heterotrimers was related to the number of the or ' subunit. The thermalstabilities of the heterotrimers were mainly conferred by the subunit which had lower thermal stability.Solubilities at low ionic strength ( = 0.08) of the heterotrimers containing the or ' subunit werevery similar to those of the and ' homotrimers, respectively. Emulsifying abilities and heat-inducedassociations of the heterotrimers containing one subunit were similar to those of the or 'homotrimer, whereas those of the heterotrimers containing two subunits were similar to those ofthe homotrimer.Keywords: -Conglycinin; structure-physicochemical function relationship; solubility; thermal stability;soybean