Electric-Field-Induced Changes in Absorption and Fluorescence of the Green Fluorescent Protein Chromophore in a PMMA Film

详细信息    查看全文

• 作者：Takakazu Nakabayashi ; Kazuyuki Hino ; Yuka Ohta ; Sayuri Ito ; Hirofumi Nakano ; Nobuhiro Ohta
• 刊名：Journal of Physical Chemistry B
• 出版年：2011
• 出版时间：July 7, 2011
• 年：2011
• 卷：115
• 期：26
• 页码：8622-8626
• 全文大小：809K
• 年卷期：v.115,no.26(July 7, 2011)
• ISSN：1520-5207

文摘

External electric field effects on absorption, fluorescence, and fluorescence decay of p-HBDI that is a model compound of the chromophore of GFP have been examined in a poly(methyl methacrylate) film. The electroabsorption spectrum is similar in shape to the first derivative of the absorption spectrum, which results from the difference in molecular polarizability between the ground state and the Franck鈥揅ondon excited state. The electrophotoluminescence spectrum is dominated by the corresponding fluorescence spectrum, indicating the enhancement of the fluorescence intensity in the presence of external electric fields. The direct measurements of the electric field effect on the fluorescence decay profile suggest that the field-induced deceleration of the nonradiative process contributes to the increase in the fluorescence intensity in the presence of electric fields.