Isolation and characterization o
f lea
f volatiles in
Anemopsis californica (Nutt.) Hook. and Arn. (
A.californica) was per
formed using steam distillation, solid-phase microextraction, and supercritical
fluidextraction. Thirty-eight compounds were detected and identi
fied by gas chromatography; elemicinwas the major component o
f the lea
f volatiles. While the composition o
f the lea
f volatiles varied withmethod o
f extraction,
fchars/alpha.gi
f" BORDER=0>-pinene, sabinene,
fchars/beta2.gi
f" BORDER=0 ALIGN="middle">-phellandrene, 1,8-cineole, piperitone, methyl eugenol,(
E)-caryophyllene, and elemicin were usually present in readily detectable amounts. Greenhouse-reared clones o
f a wild population o
f A. californica had an identical lea
f volatile composition with theparent plants. Steam-distilled oil had antimicrobial properties against 3 (
Staphylococcus aureus,
Streptococcus pneumoniae, and
Geotrichim candidum) o
f 11 microbial species tested. Some o
f thisbioactivity could be accounted
for by the
fchars/alpha.gi
f" BORDER=0>-pinene in the oil.Keywords:
Anemopsis californica, Yerba mansa, steam distillation, SPME, SFE, antimicrobial, elemicin,methyl eugenol