Ethanol-Induced Reorganization of the Liquid-Ordered Phase: Enhancement of Cholesterol-Phospholipid Association
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- 作者：Jianbing Zhang ; Honghua Cao ; Bingwen Jing ; and Steven L. Regen
- 刊名：Journal of the American Chemical Society
- 出版年：2006
- 出版时间：January 11, 2006
- 年：2006
- 卷：128
- 期：1
- 页码：265 - 269
- 全文大小：62K
- 年卷期：v.128,no.1(January 11, 2006)
- ISSN：1520-5126
文摘
This paper records what is believed to be the first evidence for the reorganization of the liquid-ordered phase by ethanol. Specifically, ethanol has been found to significantly enhance sterol-phospholipidassociation in liquid-ordered bilayers derived from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) pluscholesterol and also 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) plus cholesterol. The evidencefor such reorganization comes from a series of nearest-neighbor recognition (NNR) experiments that havebeen carried out, where low concentrations of equilibrating lipid dimers (i.e., "reporter molecules") havebeen used to detect changes in the phase composition of host membranes made from varying mixtures ofDPPC/cholesterol, and also DSPC/cholesterol, in the presence and in the absence of ethanol. These findingshave important biological implications, which are briefly discussed.