Gold Nanorod Bioconjugates
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- 作者：Hongwei Liao and Jason H. Hafner
- 刊名：Chemistry of Materials
- 出版年：2005
- 出版时间：September 6, 2005
- 年：2005
- 卷：17
- 期：18
- 页码：4636 - 4641
- 全文大小：370K
- 年卷期：v.17,no.18(September 6, 2005)
- ISSN：1520-5002
文摘
Gold nanorods have been stabilized, conjugated to antibodies, and characterized for biologicalapplications. The stabilizing surfactant bilayer which surrounds gold nanorods was replaced by thiolterminated methoxypoly(ethylene glycol) so that the nanorods are stable in buffer solutions free ofsurfactant. Nanorod bioconjugation was accomplished with a heterobifunctional cross-linker, with antibodyactivity confirmed by a strip plate assay. Nanorod bioconjugates were characterized by independentmeasurements of the nanorod and antibody concentrations. The nanorod molar extinction coefficientwas measured from dense yet well separated nanorod films to be 4.4 ± 0.5 × 109 M-1 cm-1, thus allowingquantitative determination of nanorod concentrations. However, a colorimetric protein assay overestimatedthe antibody concentration and is therefore likely perturbed by the presence of the nanorodselectromagnetically and/or chemically.