Analysis of Whole Bacterial Cells by Flow Field-Flow Fractionation and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
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- 作者：Hookeun Lee ; S. Kim Ratanathanawongs Williams ; Karen L. Wahl ; and Nancy B. Valentine
- 刊名：Analytical Chemistry
- 出版年：2003
- 出版时间：June 1, 2003
- 年：2003
- 卷：75
- 期：11
- 页码：2746 - 2752
- 全文大小：164K
- 年卷期：v.75,no.11(June 1, 2003)
- ISSN：1520-6882
文摘
The purpose of this study is to develop a novel bacterialanalysis method by coupling the flow field-flow fractionation (flow FFF) separation technique with detection bymatrix-assisted laser desorption/ionization time-of-flight(MALDI-TOF) mass spectrometry. The composition ofcarrier liquid used for flow FFF was selected based onretention of bacterial cells and compatibility with theMALDI process. The coupling of flow FFF and MALDI-TOF MS was demonstrated for P. putida and E. coli.Fractions of the whole cells were collected after separationby FFF and further analyzed by MALDI-MS. Each fraction,collected over different time intervals, corresponded todifferent sizes and possibly different growth stages ofbacteria. The bacterial analysis by flow FFF/MALDI-TOFMS was completed within 1 h with only preliminaryoptimization of the process.