Isolation and Identification of an Antiproliferative Compound from Fructose–Tryptophan Maillard Reaction Products

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• 作者：Sang Hoon Lee ; Su Jeong Jeong ; Gwi Yeong Jang ; Min Young Kim ; In Guk Hwang ; Hyun Young Kim ; Koan Sik Woo ; Bang Yeon Hwang ; Jin Song ; Junsoo Lee ; Heon Sang Jeong
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：April 20, 2016
• 年：2016
• 卷：64
• 期：15
• 页码：3041-3047
• 全文大小：411K
• 年卷期：0
• ISSN：1520-5118

文摘

This study was performed to isolate and identify a compound with antiproliferative activity against human stomach cancer cell lines, from fructose–tryptophan Maillard reaction products (MRPs). The MRPs, prepared from a fructose–tryptophan solution heated at 130 °C for 2 h, were fractionated into five solvent fractions: n-hexane, chloroform, ethyl acetate, butanol, and water. The highest antiproliferative activity was found in the chloroform fraction (85.93% at 200 μg/mL), and the active compound from this chloroform fraction was purified by silica gel column chromatography, TLC, and preparative HPLC. The antiproliferative activity (IC₅₀) of the active compound was 42.24 μg/mL, and the active compound was identified as perlolyrine (C₁₆H₁₀N₂O₂) by ¹H/¹³C NMR, DEPT, HMBC, and LC-ESI-MS. Therefore, this research may be useful in developing perlolyrine as a functional therapeutic agent.