Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells

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• 作者：Samuel J. Lord ; Hsiao-lu D. Lee ; W. E. Moerner
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：March 15, 2010
• 年：2010
• 卷：82
• 期：6
• 页码：2192-2203
• 全文大小：374K
• 年卷期：v.82,no.6(March 15, 2010)
• ISSN：1520-6882

文摘

The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes the field of live-cell imaging of single biomolecules.