A Photoactivatable Push−Pull Fluorophore for Single-Molecule Imaging in Live Cells
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- 作者：Samuel J. Lord ; Nicholas R. Conley ; Hsiao-lu D. Lee ; Reichel Samuel ; Na Liu ; Robert J. Twieg ; W. E. Moerner
- 刊名：Journal of the American Chemical Society
- 出版年：2008
- 出版时间：July 23, 2008
- 年：2008
- 卷：130
- 期：29
- 页码：9204 - 9205
- 全文大小：343K
- 年卷期：v.130,no.29(July 23, 2008)
- ISSN：1520-5126
文摘
We have reengineered a red-emitting dicyanomethylenedihydrofuran push−pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission.