Low-Density Lipoprotein, Collagen, and Thrombin Models Reveal that Rosemarinus officinalis L. Exhibits Potent Antiglycative Effects
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- 作者：Chiu-Lan Hsieh ; Chiung-Huei Peng ; Charng-Cherng Chyau ; Yuh-Charn Lin ; Hui-Er Wang ; Robert Y. Peng
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：April 18, 2007
- 年：2007
- 卷：55
- 期：8
- 页码：2884 - 2891
- 全文大小：191K
- 年卷期：v.55,no.8(April 18, 2007)
- ISSN：1520-5118
文摘
Using the low-density lipoprotein (LDL), collagen, and thrombin models, we report here that therosemary extracts (REs), either the aqueous (REw) or the acetonic (REA), all possessed manyantiglycation-related features, and the effective concentrations required were as follows: 0.1 mg/mLfor suppressing the relative electrophoretic mobility, 1.3 
g/mL for anticonjugated diene induction,0.5 mg/mL for inhibition of thiobarbituric acid reactive substances production, 0.1 mg/mL for AGEs(advanced glycation end products) formation, 0.1 mg/mL to block glucose incorporation, and 0.05mg/mL as an effective anti-antithrombin III. Using high-performance liquid chromatography/massspectrometry, we identified five major constituents among eight major peaks, including rosmarinicacid, carnosol, 12-methoxycarnosic acid, carnosic acid, and methyl carnosate. In the LDL model,REA was proven to be more efficient than REw; yet, the reverse is true for the collagen and thethrombin III models, the reason of which was ascribed to the higher lipid-soluble antioxidant content(such as rosmarinic acid, carnosol, carnosic acid, 12-methoxycarnosic acid and methyl carnosate) inREA than in REw and the different surface lipid characteristics between LDL and collagen; althoughto act as anti-AGEs, both extracts were comparable. To assist the evidence, a larger 2,2-diphenyl-1-picrylhydrazyl radical scavenging capability with less total polyphenolic content was found in REA.We conclude that rosemary is an excellent multifunctional therapeutic herb; by looking at its potentialpotent antiglycative bioactivity, it may become a good adjuvant medicine for the prevention andtreatment of diabetic, cardiovascular, and other neurodegenerative diseases.
g/mL for anticonjugated diene induction,0.5 mg/mL for inhibition of thiobarbituric acid reactive substances production, 0.1 mg/mL for AGEs(advanced glycation end products) formation, 0.1 mg/mL to block glucose incorporation, and 0.05mg/mL as an effective anti-antithrombin III. Using high-performance liquid chromatography/massspectrometry, we identified five major constituents among eight major peaks, including rosmarinicacid, carnosol, 12-methoxycarnosic acid, carnosic acid, and methyl carnosate. In the LDL model,REA was proven to be more efficient than REw; yet, the reverse is true for the collagen and thethrombin III models, the reason of which was ascribed to the higher lipid-soluble antioxidant content(such as rosmarinic acid, carnosol, carnosic acid, 12-methoxycarnosic acid and methyl carnosate) inREA than in REw and the different surface lipid characteristics between LDL and collagen; althoughto act as anti-AGEs, both extracts were comparable. To assist the evidence, a larger 2,2-diphenyl-1-picrylhydrazyl radical scavenging capability with less total polyphenolic content was found in REA.We conclude that rosemary is an excellent multifunctional therapeutic herb; by looking at its potentialpotent antiglycative bioactivity, it may become a good adjuvant medicine for the prevention andtreatment of diabetic, cardiovascular, and other neurodegenerative diseases.