Diosgenin, a Plant-Derived Sapogenin, Enhances Regulatory T-Cell Immunity in the Intestine of Mice with Food Allergy

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• 作者：Chung-Hsiung Huang ; Der-Zen Liu ; Tong-Rong Jan
• 刊名：Journal of Natural Products
• 出版年：2010
• 出版时间：June 25, 2010
• 年：2010
• 卷：73
• 期：6
• 页码：1033-1037
• 全文大小：439K
• 年卷期：v.73,no.6(June 25, 2010)
• ISSN：1520-6025

文摘

It was hypothesized that the suppressive effect of diosgenin (1) on the intestinal T helper (Th)2 responses is associated with an enhancement of the regulatory T-cell immunity. Ovalbumin (OVA)-sensitized BALB/c mice were gavaged daily with 1 and received repeatedly oral OVA challenges to induce intestinal allergic responses. The expression of Th2- and Treg-related cytokines and transcription factors was examined by immunohistochemical staining and RT-PCR. Administration of 1 markedly attenuated the intestinal expression of interleukin (IL)-4 and GATA3. In addition, administration of 1 reversed the diminished density of intestinal Foxp3⁺ cells induced by OVA oral challenges and enhanced the expression of IL-10 by Foxp3⁺ cells markedly. These results suggest that the suppressive effect of 1 on allergen-induced intestinal Th2 responses is closely associated with an up-regulation of the regulatory T-cell immunity in the inflammatory site.