C-Type Starch from High-Amylose Rice Resistant Starch Granules Modified by Antisense RNA Inhibition of Starch Branching Enzyme
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- 作者：Cunxu Wei ; Bin Xu ; Fengling Qin ; Huaguang Yu ; Chong Chen ; Xianglen Meng ; Lijia Zhu ; Youping Wang ; Minghong Gu ; Qiaoquan Liu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：June 23, 2010
- 年：2010
- 卷：58
- 期：12
- 页码：7383-7388
- 全文大小：766K
- 年卷期：v.58,no.12(June 23, 2010)
- ISSN：1520-5118
文摘
High-amylose starch is a source of resistant starch (RS) which has a great benefit on human health. A transgenic rice line (TRS) enriched amylose and RS had been developed by antisense RNA inhibition of starch branching enzymes. In this study, the native starch granules were isolated from TRS grains as well as the wild type, and their crystalline type was carefully investigated before and after acid hydrolysis. In high-amylose TRS rice, the C-type starch, which might result from the combination of both A-type and B-type starch, was observed and subsequently confirmed by multiple physical techniques, including X-ray powder diffraction, solid-state nuclear magnetic resonance, and Fourier transform infrared. Moreover, the change of starch crystalline structure from C- to B-type during acid hydrolysis was also observed in this RS-rich rice. These data could add to our understanding of not only the polymorph structure of cereal starch but also why high-amylose starch is more resistant to digestion.