NF-E2 Related Factor 2 Activation and Heme Oxygenase-1 Induction by tert-Butylhydroquinone Protect against Deltamethrin-Mediated Oxidative Stress in PC12 Cells
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- 作者：Huang Yuan Li ; Yu Fang Zhong ; Si Ying Wu ; Nian Shi
- 刊名：Chemical Research in Toxicology
- 出版年：2007
- 出版时间：September 2007
- 年：2007
- 卷：20
- 期：9
- 页码：1242 - 1251
- 全文大小：2728K
- 年卷期：v.20,no.9(September 2007)
- ISSN：1520-5010
文摘
Recent findings suggest that oxidative stress caused by pyrethroid pesticides could be closely involved in the neurotoxicity. tert-Butylhydroquinone (tBHQ) is a known inducer of Nrf2-mediated transcription, and treatment of cells with tBHQ can confer protection against H2O2 and 6-hydroxydopamine (6-OHDA). In this study, we investigated the neuroprotective effect of tBHQ against deltamethrin (DM)-induced oxidative stress using rat PC12 adrenal pheochromocytoma cells. The pretreatment of PC12 cells with tBHQ significantly reduced DM-induced generation of reactive oxygen species (ROS) and increased intracellular ionized calcium ([Ca2+]i). We also observed that DM or tBHQ induced the expression of NF-E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), a Nrf2-regulated gene. In addition, the Nrf2 antioxidant responsive element (ARE) pathways activated by tBHQ caused a partial inhibition of the DM-induced Nrf2 and HO-1 expression. Altogether, our data clearly indicate that an activation of Nrf2/ARE pathways in PC12 cells by tBHQ treatment protects cells from DM-induced oxidative stress and regulates DM- mediated adaptive responses in PC12 cells via translocation of Nrf2.