Determination of 49 Organophosphorus Pesticide Residues and Their Metabolites in Fish, Egg, and Milk by Dual Gas Chromatography鈥揇ual Pulse Flame Photometric Detection with Gel Permeation Chromatograph
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- 作者：Lixin Yang ; Heli Li ; Fangang Zeng ; Yinping Liu ; Ruifeng Li ; Huijing Chen ; Yunfeng Zhao ; Hong Miao ; Yongning Wu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：February 29, 2012
- 年：2012
- 卷：60
- 期：8
- 页码：1906-1913
- 全文大小：238K
- 年卷期：v.60,no.8(February 29, 2012)
- ISSN：1520-5118
文摘
A new method for the quantitative determination of 49 kinds of organophosphorus pesticide residues and their metabolites in fish, egg, and milk by dual gas chromatography鈥揹ual pulse flame photometric detection was developed. Homogenized samples were extracted with acetone and methylene chloride (1 + 1, v/v), and then the extracts were cleaned up by gel permeation chromatography (GPC). The response of each organophosphorus pesticide showed a good linearity with its concentration; the linearity correlation was not less than 0.99. The detection limits (S/N = 3) of pesticides were in the range of 0.001鈥?.025 mg kg鈥?. The recovery experiments were performed by blank sample spiked at low, medium, and high fortification levels. The recoveries for fish, egg, and milk were 50.9鈥?42.2, 53.3鈥?37.2, and 50.3鈥?39.4% with relative standard deviations (RSD, n = 6) of 2.3鈥?4.9, 4.3鈥?6.7, and 2.8鈥?2.2%, respectively. The method was applied to detect organophosphorus pesticides in samples collected from the market, and satisfactory results were obtained. This quantitative method was highly sensitive and exact and could be applied to the accurate determination of organophosphorus contaminants in fish, egg, and milk.