Study of Thioflavin-T Immobilized in Porous Silicon and the Effect of Different Organic Vapors on the Fluorescence Lifetime

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• 作者：Tanya Hutter ; Nadav Amdursky ; Rinat Gepshtein ; Stephen R. Elliott ; Dan Huppert
• 刊名：Langmuir
• 出版年：2011
• 出版时间：June 21, 2011
• 年：2011
• 卷：27
• 期：12
• 页码：7587-7594
• 全文大小：998K
• 年卷期：v.27,no.12(June 21, 2011)
• ISSN：1520-5827

文摘

Steady-state and time-resolved emission techniques have been employed to study the fluorescence properties of thioflavin-T (ThT) adsorbed on oxidized porous silicon (PSi) surfaces, with an average pore size of 10 nm. We found that the average fluorescence decay time of ThT, when it is adsorbed on the PSi surface, is rather long, 蟿_av = 1.3 ns. We attribute this relatively long emission lifetime to the effect of the immobilization of ThT on the PSi surface, which inhibit the rotation of the aniline with respect to the benzothiazole moieties of ThT. We also measured the fluorescence properties of ThT in PSi samples in equilibrium with vapors of several liquids, such as methanol, acetonitrile, and water. We found that the fluorescence intensity drops by a factor of 10, and the average decay time, measured by a time-correlated single-photon counting technique, decreases by a factor of 3. We explain these results in terms of liquid condensation of the vapors in the PSi pores, which leads to partial dissolution of the ThT molecules in the liquid pools.