Thin Film Assembly of Spider Silk-like Block Copolymers

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• 作者：Sreevidhya T. Krishnaji ; Wenwen Huang ; Olena Rabotyagova ; Eugenia Kharlampieva ; Ikjun Choi ; Vladimir V. Tsukruk ; Rajesh Naik ; Peggy Cebe ; David L. Kaplan
• 刊名：Langmuir
• 出版年：2011
• 出版时间：February 1, 2011
• 年：2011
• 卷：27
• 期：3
• 页码：1000-1008
• 全文大小：1286K
• 年卷期：v.27,no.3(February 1, 2011)
• ISSN：1520-5827

文摘

We report the self-assembly of monolayers of spider silk-like block copolymers. Langmuir isotherms were obtained for a series of bioengineered variants of the spider silks, and stable monolayers were generated. Langmuir鈭払lodgett films were prepared by transferring the monolayers onto silica substrates and were subsequently analyzed by atomic force microscopy (AFM). Static contact angle measurements were performed to characterize interactions across the interface (thin film, water, air), and molecular modeling was used to predict 3D conformation of spider silk-like block copolymers. The influence of molecular architecture and volume fraction of the proteins on the self-assembly process was assessed. At high surface pressure, spider silk-like block copolymers with minimal hydrophobic block (f_A = 12%) formed oblate structures, whereas block copolymer with a 6-fold larger hydrophobic domain (f_A = 46%) formed prolate structures. The varied morphologies obtained with increased hydrophobicity offer new options for biomaterials for coatings and related options. The design and use of bioengineered protein block copolymers assembled at air鈭抴ater interfaces provides a promising approach to compare 2D microstructures and molecular architectures of these amphiphiles, leading to more rationale designs for a range of nanoengineered biomaterial needs as well as providing a basis of comparison to more traditional synthetic block copolymer systems.