Preparation of Silica-Encapsulated Hollow Gold Nanosphere Tags Using Layer-by-Layer Method for Multiplex Surface-Enhanced Raman Scattering Detection

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• 作者：Jianshe Huang ; Ki Hyung Kim ; Namhyun Choi ; Hyangah Chon ; Sangyeop Lee ; Jaebum Choo
• 刊名：Langmuir
• 出版年：2011
• 出版时间：August 16, 2011
• 年：2011
• 卷：27
• 期：16
• 页码：10228-10233
• 全文大小：862K
• 年卷期：v.27,no.16(August 16, 2011)
• ISSN：1520-5827

文摘

The use of silica shells offers many advantages in surface-enhanced Raman scattering (SERS)-based biological sensing applications due to their optical transparency, remarkable stability in environmental media, and improved biocompatibility. Here, we report a novel layer-by-layer method for the preparation of silica-hollow gold nanosphere (HGN) SERS tags. Poly(acrylic acid) was used to stabilize Raman reporter-tagged HGNs prior to the adsorption of a coupling agent, after which a silica shell was deposited onto the particle surface using St枚ber鈥檚 method. Importantly, competitive adsorption of the Raman reporter molecules and coupling agents, which results in unbalanced loading of reporter molecules on individual nanoparticles, was avoided using this method. As a result, the loading density of reporter molecules could be maximized. In addition, HGNs exhibited strong enhancement effects from the individual particles because of their ability to localize the surface electromagnetic fields through pinholes in the hollow particle structures. The proposed layer-by-layer silica-encapsulated HGN tags showed strong SERS signals as well as excellent multiplexing capabilities.