Discovery and Characterization of the Tuberculosis Drug Lead Ecumicin
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- 作者：Wei Gao ; Jin-Yong Kim ; Shao-Nong Chen ; Sang-Hyun Cho ; Jongkeun Choi ; Birgit U. Jaki ; Ying-Yu Jin ; David C. Lankin ; Ji-Ean Lee ; Sun-Young Lee ; James B. McAlpine ; Jos茅 G. Napolitano ; Scott G. Franzblau ; Joo-Won Suh ; Guido F. Pauli
- 刊名：Organic Letters
- 出版年：2014
- 出版时间：December 5, 2014
- 年：2014
- 卷：16
- 期：23
- 页码：6044-6047
- 全文大小：313K
- ISSN：1523-7052
文摘
The new tuberculosis (TB) lead ecumicin (1), a cyclic tridecapeptide, was isolated from Nonomuraea sp. MJM5123, following a high-throughput campaign for anti-TB activity. The large molecular weight of 1599 amu detected by LC-HR-MS precluded the initial inference of its molecular formula. The individual building blocks were identified by extensive NMR experiments. The resulting two possible planar structures were distinguished by LC-MS2. Determination of absolute configuration and unambiguous structural confirmation were carried out by X-ray crystallography and Marfey鈥檚 analysis.