Protein Nanoparticles Engineered to Sense Kinase Activity in MRI

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• 作者：Mikhail G. Shapiro ; Jerzy O. Szablowski ; Robert Langer ; Alan Jasanoff*
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：February 25, 2009
• 年：2009
• 卷：131
• 期：7
• 页码：2484-2486
• 全文大小：206K
• 年卷期：v.131,no.7(February 25, 2009)
• ISSN：1520-5126

文摘

We introduce a family of protein nanoparticles capable of sensing analytes in conjunction with magnetic resonance imaging (MRI). The new sensors are derived from the iron storage protein ferritin (Ft); they are designed and optimized using facile protein engineering methods, and self-assembled in cells harboring specific combinations of DNA coding sequences. As illustration, we show that suitably constructed Ft-based sensors can report activity of the important neural signaling enzyme protein kinase A (PKA). Phosphorylation of the engineered Ft-based nanoparticles by PKA promotes clustering and changes in T₂-weighted MRI signal.