A Locked Nucleic Acid-Based Nanocrawler: Designed and Reversible Movement Detected by Multicolor Fluorescence

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• 作者：I. Kira Astakhova ; Karol Pasternak ; Meghan A. Campbell ; Pankaj Gupta ; Jesper Wengel
• 刊名：The Journal of the American Chemical Society
• 出版年：2013
• 出版时间：February 20, 2013
• 年：2013
• 卷：135
• 期：7
• 页码：2423-2426
• 全文大小：295K
• 年卷期：v.135,no.7(February 20, 2013)
• ISSN：1520-5126

文摘

Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2鈥?amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene鈥損erylene interstrand F枚rster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.