Real-Time Evaluation of Live Cancer Cells by an in Situ Surface Plasmon Resonance and Electrochemical Study

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• 作者：Changyu Wu ; Fawad ur Rehman ; Jingyuan Li ; Jing Ye ; Yuanyuan Zhang ; Meina Su ; Hui Jiang ; Xuemei Wang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：November 11, 2015
• 年：2015
• 卷：7
• 期：44
• 页码：24848-24854
• 全文大小：460K
• ISSN：1944-8252

文摘

This work presents a new strategy of the combination of surface plasmon resonance (SPR) and electrochemical study for real-time evaluation of live cancer cells treated with daunorubicin (DNR) at the interface of the SPR chip and living cancer cells. The observations demonstrate that the SPR signal changes could be closely related to the morphology and mass changes of adsorbed cancer cells and the variation of the refractive index of the medium solution. The results of light microscopy images and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide studies also illustrate the release or desorption of HepG2 cancer cells, which were due to their apoptosis after treatment with DNR. It is evident that the extracellular concentration of DNR residue can be readily determined through electrochemical measurements. The decreases in the magnitudes of SPR signals were linearly related to cell survival rates, and the combination of SPR with electrochemical study could be utilized to evaluate the potential therapeutic efficiency of bioactive agents to cells. Thus, this label-free, real-time SPR鈥揺lectrochemical detection technique has great promise in bioanalysis or monitoring of relevant treatment processes in clinical applications.