A gene-centric Hu
man Proteo
me Project has been proposed to characterize the hu
man protein-coding genes in a chro
moso
me-centered
manner to understand hu
man biology and disease. Here, we report on the protein evidence for all genes predicted fro
m the geno
me sequence based on
manual annotation fro
m literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in hu
man cell lines of different origins. We esti
mate that there is good evidence for protein existence for 69% (
n = 13985) of the hu
man protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experi
mental evidence. Analysis of the expression patterns shows few tissue-specific proteins and approxi
mately half of the genes expressed in all the analyzed cells. The status for each gene with regards to protein evidence is visualized in a chro
moso
me-centric
manner as part of a new version of the Hu
man Protein Atlas (
www.proteinatlas.org).
Keywords:
Antibody-based protein profiling; C-HPP