Transmission FT-IR Chemical Imaging on Glass Substrates: Applications in Infrared Spectral Histopathology

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• 作者：Paul Bassan ; Joe Mellor ; Jonathan Shapiro ; Kaye J Williams ; Michael P. Lisanti ; Peter Gardner
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：February 4, 2014
• 年：2014
• 卷：86
• 期：3
• 页码：1648-1653
• 全文大小：484K
• ISSN：1520-6882

文摘

Fourier transform-infrared (FT-IR) chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass, making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FT-IR chemical imaging, which has a high-wavenumber transmission window allowing measurement of the C鈥揌, N鈥揌, and O鈥揌 stretches occurring at ca. 2500鈥?800 cm^鈥?. The 鈥渇ingerprint鈥?region of the IR spectrum occurring below 1800 cm^鈥? is not obtainable; however, we demonstrate that a wealth of information is contained in the high wavenumber range using 71 patients on a breast tissue microarray (TMA) as a model for investigation. Importantly, we demonstrate that the tissue can be classified into four basic tissue cell types and that using just the epithelial cells, reasonable discrimination of normal and malignant tissue can be found.