High-Throughput Quantification of Bioactive Lipids by MALDI Mass Spectrometry: Application to Prostaglandins

详细信息    查看全文

• 作者：Joseph D. Manna ; Michelle L. Reyzer ; Joey C. Latham ; C. David Weaver ; Lawrence J. Marnett ; Richard M. Caprioli
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：September 1, 2011
• 年：2011
• 卷：83
• 期：17
• 页码：6683-6688
• 全文大小：839K
• 年卷期：v.83,no.17(September 1, 2011)
• ISSN：1520-6882

文摘

Analysis and quantification of analytes in biological systems is a critical component of metabolomic investigations of cell function. The most widely used methods employ chromatographic separation followed by mass spectrometric analysis, which requires significant time for sample preparation and sequential chromatography. We introduce a novel high-throughput, separation-free methodology based on MALDI mass spectrometry that allows for the parallel analysis of targeted metabolomes. Proof-of-concept is demonstrated by analysis of prostaglandins and glyceryl prostaglandins. Derivatization to incorporate a charged moiety into ketone-containing prostaglandins dramatically increases the signal-to-noise ratio relative to underivatized samples. This resulted in an increased dynamic range (15鈥?000 fmol on plate) and improved linearity (r² = 0.99). The method was adapted for high-throughput screening methods for enzymology and drug discovery. Application to cellular metabolomics was also demonstrated.