Aryl Azide Photochemistry in Defined Protein Environments

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• 作者：Josephine L. Morris ; Samuel C. Reddington ; Damien M. Murphy ; D. Dafydd Jones ; James A. Platts ; Eric M. Tippmann
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：February 15, 2013
• 年：2013
• 卷：15
• 期：4
• 页码：728-731
• 全文大小：255K
• 年卷期：v.15,no.4(February 15, 2013)
• ISSN：1523-7052

文摘

A genetically encoded precursor to an aryl nitrene, para-azidophenylalanine, was introduced site specifically into proteins to deduce if distinct environments were capable of caging a reactive organic intermediate. Following photolysis of mutant T4 lysozyme or green fluorescent proteins, EPR spectra showed, respectively, the presence of a triplet nitrene and an anilino radical.