Sulfonated Polyethylenimine for Photosensitizer Conjugation and Targeting

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• 作者：Upendra Chitgupi ; Yumiao Zhang ; Chi Y. Lo ; Shuai Shao ; Wentao Song ; Jumin Geng ; Sriram Neelamegham ; Jonathan F. Lovell
• 刊名：Bioconjugate Chemistry
• 出版年：2015
• 出版时间：August 19, 2015
• 年：2015
• 卷：26
• 期：8
• 页码：1633-1639
• 全文大小：366K
• ISSN：1520-4812

文摘

Polysulfonated macromolecules are known to bind selectins, adhesion membrane proteins which are broadly implicated in inflammation. Commercially available branched polyethylenimine (PEI) was reacted with chlorosulfonic acid to generate sulfonated PEI with varying degrees of sulfonation. Remaining unreacted amine groups were then used for straightforward conjugation with pyropheophoribide-a, a near-infrared photosensitizer. Photosensitizer-labeled sulfonated PEI conjugates inhibited blood coagulation and were demonstrated to specifically bind to cells genetically programmed to overexpress L-selectin (CD62L) or P-selectin (CD62P). In vitro, following targeting, selectin-expressing cells could be destroyed via photodynamic therapy.