Cellular Engineering with Membrane Fusogenic Liposomes to Produce Functionalized Extracellular Vesicles
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- 作者：Junsung Lee ; Hyoungjin Lee ; Unbyeol Goh ; Jiyoung Kim ; Moonkyoung Jeong ; Jean Lee ; Ji-Ho Park
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2016
- 出版时间：March 23, 2016
- 年：2016
- 卷：8
- 期：11
- 页码：6790-6795
- 全文大小：414K
- ISSN：1944-8252
文摘
Engineering of extracellular vesicles (EVs) without affecting biological functions remains a challenge, limiting the broad applications of EVs in biomedicine. Here, we report a method to equip EVs with various functional agents, including fluorophores, drugs, lipids, and bio-orthogonal chemicals, in an efficient and controlled manner by engineering parental cells with membrane fusogenic liposomes, while keeping the EVs intact. As a demonstration of how this method can be applied, we prepared EVs containing azide-lipids, and conjugated them with targeting peptides using copper-free click chemistry to enhance targeting efficacy to cancer cells. We believe that this liposome-based cellular engineering method will find utility in studying the biological roles of EVs and delivering therapeutic agents through their innate pathway.