Soybean Toxin (SBTX), a Protein from Soybeans That Inhibits the Life Cycle of Plant and Human Pathogenic Fungi
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- 作者：Janne Keila S. Morais ; Valdirene M. Gomes ; Jos Tadeu A. Oliveira ; Izabela S. Santos ; Maura Da Cunha ; Hermogenes D. Oliveira ; Henrique P. Oliveira ; Daniele O. B. Sousa ; Ilka M. Vasconcelos
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：October 13, 2010
- 年：2010
- 卷：58
- 期：19
- 页码：10356-10363
- 全文大小：1013K
- 年卷期：v.58,no.19(October 13, 2010)
- ISSN：1520-5118
文摘
Soybean toxin (SBTX) is a 44 kDa glycoprotein that is lethal to mice (LD50 = 5.6 mg/kg). This study reports the toxicity of SBTX on pathogenic fungi and yeasts and the mechanism of its action. SBTX inhibited spore germination of Aspergillus niger and Penicillium herguei and was toxic to Candida albicans, Candida parapsilosis, Kluyveromyces marxiannus, Pichia membranifaciens, and Saccharomyces cerevisiae. In addition, SBTX hampered the growth of C. albicans and K. marxiannus and inhibited the glucose-stimulated acidification of the incubation medium by S. cerevisiae, suggesting that SBTX interferes with intracellular proton transport to the external medium. Moreover, SBTX caused cell-wall disruption, condensation/shrinkage of cytosol, pseudohyphae formation, and P. membranifaciens and C. parapsilosis cell death. SBTX is toxic to fungi at concentrations far below the dose lethal to mice and has potential in the design of new antifungal drugs or in the development of transgenic crops resistant to pathogens.