Recent progress in metabolomics and t
he development of increasingly sensitive analytical tec
hniques
have renewed interest in global profiling, i.e., semiquantitative monitoring of all c
hemical constituents of biological fluids. In t
his work, we
have performed global profiling of NIST SRM 1950, 鈥淢etabolites in Human Plasma鈥? using GC-MS, LC-MS, and NMR. Metabolome coverage, difficulties, and reproducibility of t
he experiments on eac
h platform are discussed. A total of 353 metabolites
have been identified in t
his material. GC-MS provides 65 unique identifications, and most of t
he identifications from NMR overlap wit
h t
he LC-MS identifications, except for some small sugars t
hat are not directly found by LC-MS. Also, repeatability and intermediate precision analyses s
how t
hat t
he SRM 1950 profiling is reproducible enoug
h to consider t
his material as a good c
hoice to distinguis
h between analytical and biological variability. Clinical laboratory data s
hows t
hat most results are wit
hin t
he reference ranges for eac
h assay. In-
house computational tools
have been developed or modified for MS data processing and interactive web display. All data and programs are freely available online at
href="http://peptide.nist.gov/" class="extLink">http://peptide.nist.gov/ and href="http://srmd.nist.gov/" class="extLink">http://srmd.nist.gov/.