文摘
Gallic acid is one of the main phenolic components of black tea. The objective of this study was toidentify urinary gallic acid metabolites with potential for use as markers of black tea intake. In aninitial study, nine compounds, assessed by using gas chromatography-mass spectrometry, werefound to increase in concentration in urine after 3 cups of black tea over 3 h. A subsequent studyemployed a controlled crossover design in which 10 subjects consumed 5 cups per day of black teaor water for 4 weeks in random order. Twenty-four hour urine samples were collected at the end ofeach period. Of the 9 candidate compounds identified in the initial study, only 3 were present athigher concentrations in urine of all 10 subjects during tea-drinking in comparison to water-drinkingperiods. These compounds were identified as 4-O-methylgallic acid, 3-O-methylgallic acid, and 3,4-O-dimethylgallic acid, all methyl ether derivatives of gallic acid. It is suggested that these compoundshave the potential to be used as markers of black tea intake.Keywords: Black tea; gallic acid; 4-O-methylgallic acid; 3-O-methylgallic acid; 3,4-O-dimethylgallicacid