Structure of the Major O-Specific Polysaccharide from the Lipopolysaccharide of Pseudomonas fluorescens BIM B-582: Identification of 4-Deoxy-d-xylo-hexose

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• 作者：Olga A. Valueva ; Dzianis Rakhuba ; Alexander S. Shashkov ; Evelina L. Zdorovenko ; Elena Kiseleva ; Galina Novik ; Yuriy A. Knirel
• 刊名：Journal of Natural Products
• 出版年：2011
• 出版时间：October 28, 2011
• 年：2011
• 卷：74
• 期：10
• 页码：2161-2167
• 全文大小：822K
• 年卷期：v.74,no.10(October 28, 2011)
• ISSN：1520-6025

文摘

A novel constituent of bacterial polysaccharides, 4-deoxy-d-xylo-hexose (d-4dxylHex), was found in the major O-specific polysaccharide from the lipopolysaccharide of Pseudomonas fluorescens BIM B-582. d-4dxylHex was isolated in the free state by paper chromatography after full acid hydrolysis of the polysaccharide and identified by GLC鈥搈ass spectrometry, ¹H and ¹³C NMR spectroscopy, and specific rotation. It occurs as a lateral substituent in 40% of the oligosaccharide repeating units, making the polysaccharide devoid of strict regularity. The structure of the polysaccharide was established by sugar analysis, Smith degradation, and two-dimensional ¹H and ¹³C NMR spectroscopy. In addition, a minor polysaccharide was isolated from the same lipopolysaccharide and found to contain 4-O-methylrhamnose.