Detecting the Effect of Very Low Amounts of Penetrants in Lipid Bilayers Using Raman Spectroscopy

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• 作者：Robert J. Meier ; Agnes Csiszá ; r ; Erwin Klumpp
• 刊名：Journal of Physical Chemistry B
• 出版年：2006
• 出版时间：October 26, 2006
• 年：2006
• 卷：110
• 期：42
• 页码：20727 - 20728
• 全文大小：53K
• 年卷期：v.110,no.42(October 26, 2006)
• ISSN：1520-5207

文摘

We show that, by the influence on the gauche content in the acyl chains, the effect of very low concentrations(order 10^-2 %) of penetrants (here 2,4-dichlorophenol) in lipid bilayers (here dipalmitoyl-glycerophosphatidylcholine) can be experimentally seen in the Raman spectra. The observed effects suggest increased orderand are in accordance with previous X-ray diffraction and calorimetric data. These findings show great promisefor studying in situ the effect of low amounts of various small molecules on cell membranes using Ramanspectroscopy.