Visualization of Phospholipid Particle Fusion Induced by Duramycin

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• 作者：Soichiro Matsunaga ; Takuro Matsunaga ; Kunihiko Iwamoto ; Taro Yamada ; Mitsuhiro Shibayama ; Maki Kawai ; Toshihide Kobayashi
• 刊名：Langmuir
• 出版年：2009
• 出版时间：July 21, 2009
• 年：2009
• 卷：25
• 期：14
• 页码：8200-8207
• 全文大小：1010K
• 年卷期：v.25,no.14(July 21, 2009)
• ISSN：1520-5827

文摘

We visualized nanometer-scale phospholipid particle fusion by scanning tunneling microscopy (STM) on an alkanethiol-modified gold substrate, induced by duramycin, a tetracyclic antibiotic peptide with 19 amino residues. Ultrasonic homogenization generated a suspension mainly consisting of minimal lipid particles (MLP) from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) in a phosphate buffer solution, confirmed by dynamic light scattering (DLS). In situ STM discerned individual MLP as particles (diameter 8 nm) spread on Au(111), modified with alkanethiol, within the suspension. The MLP became fragile by the presence of duramycin, and the MLP were easily scratched by the scanning tip into multilayers along the surface. This process of particle fusion on the gold surface coincides with the aggregation of MLP in the suspension, observed by DLS. It was demonstrated that STM is capable of discerning and monitoring the nanometer-scale features of phospholipid particles altered by antibiotics with biochemical impact. STM might allow in situ, real-space, nanometer-scale observations of minute particles composed of phospholipids within the real cells with the highest magnification ratio.