Bacillus subtilis Biosensor Engineered To Assess Meat Spoilage

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• 作者：Alicja Daszczuk ; Yonathan Dessalegne ; Isma锚l Drenth ; Elbrich Hendriks ; Emeraldo Jo ; Tom van Lente ; Arjan Oldebesten ; Jonathon Parrish ; Wlada Poljakova ; Annisa A. Purwanto ; Renske van Raaphorst ; Mirjam Boonstra ; Auke van Heel ; Martijn Herber ; Sjoerd van der Meulen ; Jeroen Siebring ; Robin A. Sorg ; Matthias Heinemann ; Oscar P. Kuipers ; Jan-Willem Veening
• 刊名：ACS Synthetic Biology
• 出版年：2014
• 出版时间：December 19, 2014
• 年：2014
• 卷：3
• 期：12
• 页码：999-1002
• 全文大小：191K
• ISSN：2161-5063

文摘

Here, we developed a cell-based biosensor that can assess meat freshness using the Gram-positive model bacterium Bacillus subtilis as a chassis. Using transcriptome analysis, we identified promoters that are specifically activated by volatiles released from spoiled meat. The most strongly activated promoter was P_sboA, which drives expression of the genes required for the bacteriocin subtilosin. Next, we created a novel BioBrick compatible integration plasmid for B. subtilis and cloned P_sboA as a BioBrick in front of the gene encoding the chromoprotein amilGFP inside this vector. We show that the newly identified promoter could efficiently drive fluorescent protein production in B. subtilis in response to spoiled meat and thus can be used as a biosensor to detect meat spoilage.