Production of Tripeptide from Gelatin Using Collagenase-Immobilized Porous Hollow-Fiber Membrane

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• 作者：Ayako Fujita ; Hidetaka Kawakita ; Kyoichi Saito ; Kazuyuki Sugita ; Masao Tamada ; and Takanobu Sugo
• 刊名：Biotechnology Progress
• 出版年：2003
• 出版时间：August 2003
• 年：2003
• 卷：19
• 期：4
• 页码：1365 - 1367
• 全文大小：68K
• 年卷期：v.19,no.4(August 2003)
• ISSN：1520-6033

文摘

Tripeptide was produced during the permeation of a gelatin solution through the poreof a collagenase-immobilized porous hollow-fiber membrane. Gelatin was obtained viahydrolysis of fish collagen. First, an epoxy-group-containing monomer was graft-polymerized onto an electron-beam-irradiated porous hollow-fiber membrane. Second,the 2-hydroxyethylamino group was introduced into the epoxy group to bind collagenaseon the basis of electrostatic interaction. Third, adsorbed collagenase was cross-linkedwith glutaraldehyde to prevent leakage of the enzyme. Gelatin solution (10-50 g/L)was forced to permeate across the collagenase-immobilized porous hollow-fiber membrane with a density of immobilized collagenase of 52 mg/g at various residence timesof the gelatin solution ranging from 0.13 to 20 min. Fourteen percent in weight of 10g/L gelatin solution was hydrolyzed into tripeptide at a residence time of 20 min.