Effective Antifouling Using Quorum-Quenching Acylase Stabilized in Magnetically-Separable Mesoporous Silica

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• 作者：Byoungsoo Lee ; Kyung-Min Yeon ; Jongmin Shim ; Sang-Ryoung Kim ; Chung-Hak Lee ; Jinwoo Lee ; Jungbae Kim
• 刊名：Biomacromolecules
• 出版年：2014
• 出版时间：April 14, 2014
• 年：2014
• 卷：15
• 期：4
• 页码：1153-1159
• 全文大小：457K
• 年卷期：v.15,no.4(April 14, 2014)
• ISSN：1526-4602

文摘

Highly effective antifouling was achieved by immobilizing and stabilizing an acylase, disrupting bacterial cell-to-cell communication, in the form of cross-linked enzymes in magnetically separable mesoporous silica. This so-called 鈥渜uorum-quenching鈥?acylase (AC) was adsorbed into spherical mesoporous silica (S-MPS) with magnetic nanoparticles (Mag-S-MPS), and further cross-linked for the preparation of nanoscale enzyme reactors of AC in Mag-S-MPS (NER-AC/Mag-S-MPS). NER-AC effectively stabilized the AC activity under rigorous shaking at 200 rpm for 1 month, while free and adsorbed AC lost more than 90% of their initial activities in the same condition within 1 and 10 days, respectively. When applied to the membrane filtration for advanced water treatment, NER-AC efficiently alleviated the biofilm maturation of Pseudomonas aeruginosa PAO1 on the membrane surface, thereby enhancing the filtration performance by preventing membrane fouling. Highly stable and magnetically separable NER-AC, as an effective and sustainable antifouling material, has a great potential to be used in the membrane filtration for water reclamation.