Chemoselective Immobilization of Peptides on Abiotic and Cell Surfaces at Controlled Densities

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• 作者：Venkata R. Krishnamurthy ; John T. Wilson ; Wanxing Cui ; XueZheng Song ; Yi Lasanajak ; Richard D. Cummings ; Elliot L. Chaikof
• 刊名：Langmuir
• 出版年：2010
• 出版时间：June 1, 2010
• 年：2010
• 卷：26
• 期：11
• 页码：7675-7678
• 全文大小：734K
• 年卷期：v.26,no.11(June 1, 2010)
• ISSN：1520-5827

文摘

We report herein a new and enabling approach for decorating both abiotic and cell surfaces with the extracellular matrix IKVAV peptide in a site-specific manner using strain promoted azide−alkyne cycloaddition. A cyclooctyne-derivatized IKVAV peptide was synthesized and immobilized on the surface of pancreatic islets through strain-promoted azide−alkyne cycloaddition with cell surface azides generated by the electrostatic adsorption of a cytocompatible poly(l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) copolymer bearing azido groups (PP-N₃). Both “one-pot” and sequential addition of PP-N₃ and a cyclooctyne-derivatized IKVAV peptide conjugate enabled efficient modification of the pancreatic islet surface in less than 60 min. The ability to bind peptides at controlled surface densities was demonstrated in a quantitative manner using microarrays. Additionally, the technique is remarkably rapid and highly efficient, opening new avenues for the molecular engineering of cellular interfaces and protein and peptide microarrays.