Molecular Investigation of Iron鈥揝ulfur Cluster Assembly Scaffolds under Stress

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• 作者：B茅atrice Blanc ; Martin Cl茅mancey ; Jean-Marc Latour ; Marc Fontecave ; Sandrine Ollagnier de Choudens
• 刊名：Biochemistry
• 出版年：2014
• 出版时间：December 23, 2014
• 年：2014
• 卷：53
• 期：50
• 页码：7867-7869
• 全文大小：183K
• ISSN：1520-4995

文摘

Fe/S biosynthesis is controlled in Escherichia coli by two machineries, the housekeeping ISC machinery and the SUF system that is functional under stress conditions. Despite many in vivo studies showing that SUF is more adapted for Fe/S assembly under stress, no molecular data supporting this concept have been provided so far. This work focuses on molecular studies of key actors in Fe/S assembly, the SufB and IscU scaffolds under oxidative stress and iron limitation. We show that the IscU Fe₂S₂ cluster is less stable than the SufB Fe₂S₂ cluster in the presence of hydrogen peroxide, oxygen, and an iron chelator.