Reaction of PerR with Molecular Oxygen May Assist H2O2 Sensing in Anaerobes

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• 作者：Ramakrishnan Sethu ; Eric Gouré ; Luca Signor ; Christelle Caux-Thang ; Martin Clémancey ; Victor Duarte ; Jean-Marc Latour
• 刊名：ACS Chemical Biology
• 出版年：2016
• 出版时间：May 20, 2016
• 年：2016
• 卷：11
• 期：5
• 页码：1438-1444
• 全文大小：396K
• 年卷期：0
• ISSN：1554-8937

文摘

PerR is the peroxide resistance regulator found in several pathogenic bacteria and governs their resistance to peroxide stress by inducing enzymes that destroy peroxides. However, it has recently been implicated as a key component of the aerotolerance in several facultative or strict anaerobes, including the highly pathogenic Staphylococcus aureus. By combining ¹⁸O labeling studies to ESI- and MALDI-TOF MS detection and EMSA experiments, we demonstrate that the active form of PerR reacts with dioxygen, which leads ultimately to disruption of the PerR/DNA complex and is thus physiologically meaningful. Moreover, we show that the presence of O₂ assists PerR sensing of H₂O₂, another feature likely to be important for anaerobic organisms. These results allow one to envisage different scenarios for the response of anaerobes to air exposure.