The “PepSAVI-MS” Pipeline for Natural Product Bioactive Peptide Discovery

详细信息    查看全文

• 作者：Christine L. Kirkpatrick ; Christopher A. Broberg ; Elijah N. McCool ; Woo Jean Lee ; Alex Chao ; Evan W. McConnell ; David A. Pritchard ; Michael Hebert ; Renee Fleeman ; Jessie Adams ; Amer Jamil ; Laurence Madera ; Adam A. StrömstedtUlf Göransson ; Yufeng Liu ; David W. Hoskin ; Lindsey N. Shaw ; Leslie M. Hicks
• 刊名：Analytical Chemistry
• 出版年：2017
• 出版时间：January 17, 2017
• 年：2017
• 卷：89
• 期：2
• 页码：1194-1201
• 全文大小：410K
• ISSN：1520-6882

文摘

The recent increase in extensively drug-resistant bacterial pathogens and the associated increase of morbidity and mortality demonstrate the immediate need for new antibiotic backbones with novel mechanisms of action. Here, we report the development of the PepSAVI-MS pipeline for bioactive peptide discovery. This highly versatile platform employs mass spectrometry and statistics to identify bioactive peptide targets from complex biological samples. We validate the use of this platform through the successful identification of known bioactive peptides from a botanical species, Viola odorata. Using this pipeline, we have widened the known antimicrobial spectrum for V. odorata cyclotides, including antibacterial activity of cycloviolacin O2 against A. baumannii. We further demonstrate the broad applicability of the platform through the identification of novel anticancer activities for cycloviolacins by their cytotoxicity against ovarian, breast, and prostate cancer cell lines.