Switchavidin: Reversible Biotin鈥揂vidin鈥揃iotin Bridges with High Affinity and Specificity
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- 作者：Barbara Taskinen ; Dominik Zauner ; Soili I. Lehtonen ; Masi Koskinen ; Chloe Thomson ; Niklas K盲hk枚nen ; Sampo Kukkurainen ; Juha A. E. M盲盲tt盲 ; Teemu O. Ihalainen ; Markku S. Kulomaa ; Hermann J. Gruber ; Vesa P. Hyt枚nen
- 刊名：Bioconjugate Chemistry
- 出版年：2014
- 出版时间：December 17, 2014
- 年：2014
- 卷：25
- 期：12
- 页码：2233-2243
- 全文大小：517K
- ISSN：1520-4812
文摘
Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling.