Free Energy of a Polymer in Slit-like Confinement from the Odijk Regime to the Bulk
详细信息 查看全文
- 作者：Jason S. Leith ; Albert Kamanzi ; David Sean ; Daniel Berard ; Andrew C. Guthrie ; Christopher M. J. McFaul ; Gary W. Slater ; Hendrick W. de Haan ; Sabrina R. Leslie
- 刊名：Macromolecules
- 出版年：2016
- 出版时间：December 13, 2016
- 年：2016
- 卷：49
- 期：23
- 页码：9266-9271
- 全文大小：415K
- ISSN：1520-5835
文摘
We directly measure the free energy of confinement for semiflexible polymers from the nanoscale to bulk regimes in slit-like confinement. We use convex lens-induced confinement (CLiC) microscopy of DNA to directly count molecules at equilibrium in a single chamber of smoothly increasing height. Our data, acquired across a continuum of confinement regimes, provide a bridge with which to connect scaling theories established for qualitatively different regimes. We present new experimental data and simulations that connect the Odijk theory describing sub-persistence-length confinement, the interpolation model by Chen and Sullivan extending Odijk to moderate confinement, and the Casassa theory describing the transition from moderate confinement to bulk. Further, this work establishes a robust, quantitative platform for understanding and manipulating biopolymers at the nanoscale, with key applications and insights toward emerging genomic analysis tools.