Label-Free Colorimetric Detection of Gelatinases on Nanoporous Silicon Photonic Films

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• 作者：Lizeng Gao ; Njideka Mbonu ; Liangliang Cao ; Di Gao
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：March 1, 2008
• 年：2008
• 卷：80
• 期：5
• 页码：1468 - 1473
• 全文大小：325K
• 年卷期：v.80,no.5(March 1, 2008)
• ISSN：1520-6882

文摘

We report the development of a sensor platform fordetection of gelatinases based on porous silicon photonicfilms. The sensor is made by spin-coating gelatin, asubstrate protein to gelatinases, onto the porous silicon,which forms a thin, uniform, and smooth gel layer wheresamples can be directly spotted. The digestion productsof gelatin by the active gelatinase present in the sampleare able to enter the pores and induce color changes thatcan be detected by the naked eye. Using this sensor, wehave demonstrated the detection of matrix metalloproteinase-2 (MMP-2)-an important gelatinase closely associated with tumor aggressiveness and metastatic potential-with concentrations varying from 0.1 to 1000 ng/mL in samples with volumes as small as 1 L. Thedetection limit of this method, in terms of the minimumquantity of active MMP-2 in the sample that can bedetected, is 2 orders of magnitude lower than what hasbeen reported for zymography.