Polyvalent Lactose−Quantum Dot Conjugate for Fluorescent Labeling of Live Leukocytes
详细信息 查看全文
- 作者：Min Yu ; Yang Yang ; Rongcheng Han ; Qiang Zheng ; Lijun Wang ; Yuankai Hong ; Zhongjun Li ; Yinlin Sha
- 刊名：Langmuir
- 出版年：2010
- 出版时间：June 1, 2010
- 年：2010
- 卷：26
- 期：11
- 页码：8534-8539
- 全文大小：803K
- 年卷期：v.26,no.11(June 1, 2010)
- ISSN：1520-5827
文摘
Oligosaccharides play crucial roles in many biorecognition processes by the so-called “cluster glycosidic effect”. We here report a facile synthesis of lactose-CdSeS/ZnS quantum dot conjugate (Lac-QDs) by use of 1-thiol-β-d-lactose via ligand exchange, which exhibits significantly high affinity and specificity to leukocytes in contrast to the monovalent lactose. Structural analyses indicate that there are about 132 lactosyl molecules assembled on single QDs and the hydrodynamic diameter is small, close to 8.2 nm. Further, Lac-QDs display good fluorescence and physicochemical stability in physiological conditions, as well as extremely low cytotoxicity. These properties facilitate the use of Lac-QDs in fluorescent labeling of live leukocytes.