High Contrast Upconversion Luminescence Targeted Imaging in Vivo Using Peptide-Labeled Nanophosphors

详细信息    查看全文

• 作者：Liqin Xiong ; Zhigang Chen ; Qiwei Tian ; Tianye Cao ; Congjian Xu ; Fuyou Li
• 刊名：Analytical Chemistry
• 出版年：2009
• 出版时间：November 1, 2009
• 年：2009
• 卷：81
• 期：21
• 页码：8687-8694
• 全文大小：404K
• 年卷期：v.81,no.21(November 1, 2009)
• ISSN：1520-6882

文摘

Fluorescence targeted imaging in vivo has proven useful in tumor recognition and drug delivery. In the process of in vivo imaging, however, a high autofluorescence background could mask the signals from the fluorescent probes. Herein, a high contrast upconversion luminescence (UCL) imaging protocol was developed for targeted imaging of tumors based on RGD-labeled upconversion nanophosphors (UCNPs) as luminescent labels. Confocal Z-scan imaging of tissue slices revealed that UCL imaging showed no autofluorescence signal even at high penetration depth (600 μm). More importantly, region of interest (ROI) analysis of the UCL signal in vivo showed that UCL imaging achieved a high signal-to-noise ratio (24) between the tumor and the background. These results demonstrate that the UCL imaging technique appears particularly suited for applications in tracking and labeling components of complex biological systems.