Development of a Disposable Potentiometric Sensor for the Near Patient Testing of Plasma Thiol Concentrations

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• 作者：Svetlana Gracheva ; Callum Livingstone ; and James Davis
• 刊名：Analytical Chemistry
• 出版年：2004
• 出版时间：July 1, 2004
• 年：2004
• 卷：76
• 期：13
• 页码：3833 - 3836
• 全文大小：85K
• 年卷期：v.76,no.13(July 1, 2004)
• ISSN：1520-6882

文摘

The reaction of naphthoquinone with physiological thiolshas been investigated as the basis of a NPT strategy forthe measurement of the latter. Screen-printed carbonelectrode assemblies provide an inexpensive and inherently disposable platform for the detection methodology.The key analytical parameters underpinning the selectiveand sensitive (0.4 uM-1 mM) determination of reducedthiol have been assessed, and the clinical efficacy of theapproach has been demonstrated through its applicationto the analysis of human plasma. The results have beencorroborated using standard techniques, and the routesthrough which the system can be adopted within mainstream biomedical environments are highlighted.