Preparation and Characterization of Porous Horseradish Peroxidase Microspheres for the Removal of Phenolic Compound and Dye
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- 作者：Yanjun Jiang ; Cuicui Cui ; Liya Zhou ; Ying He ; Jing Gao
- 刊名：Industrial & Engineering Chemistry Research
- 出版年：2014
- 出版时间：May 7, 2014
- 年：2014
- 卷：53
- 期：18
- 页码：7591-7597
- 全文大小：396K
- 年卷期：v.53,no.18(May 7, 2014)
- ISSN：1520-5045
文摘
In this article, a simple, versatile, and mild method was used to fabricate porous enzyme microspheres using CaCO3 microparticles as templates. The preparation process involved three steps including coprecipitation of enzyme with CaCO3, cross-linking, and template removal. The preparation process was optimized and the obtained porous horseradish peroxidase (HRP) microspheres (p-HRP) were characterized. The p-HRP exhibited improved thermal stability and was more stable under alkali conditions than its free counterpart. These features are attractive for practical applications. Compared to free HRP, the Km value of p-HRP was increased and the Vmax value was decreased. The catalytic performance of p-HRP in removing phenolic compound and dye from aqueous solution was explored by using phenol and Direct Black-38 as model substrates. The results indicated that p-HRP exhibited high removal efficiency and moderately good reusability.