Poly(amido amine)-Based Mannose-Glycodendrimers As Multielectron Redox Probes for Improving Lectin Sensing

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• 作者：Manuel C. Martos-Maldonado ; Juan M. Casas-Solvas ; Indalecio Quesada-Soriano ; Luis Garc铆a-Fuentes ; Antonio Vargas-Berenguel
• 刊名：Langmuir
• 出版年：2013
• 出版时间：January 29, 2013
• 年：2013
• 卷：29
• 期：4
• 页码：1318-1326
• 全文大小：392K
• 年卷期：v.29,no.4(January 29, 2013)
• ISSN：1520-5827

文摘

An easy-to-prepare series of electroactive poly(amido amine) (PAMAM)-based dendrimers of generations G0 to G2 having mannopyranosylferrocenyl moieties in the periphery to detect carbohydrate鈥損rotein interactions is reported. The synthesis involved the functionalization of the PAMAM surface with azidomethylferrocenyl groups and subsequent coupling of mannoside units by the Cu(I)-catalyzed Huisgen reaction. The binding affinity of the series of electroactive glycodendrimers was studied by isothermal titration calorimetry (ITC) and differential pulse voltammetry (DPV). Upon complexation of the glycodendrimers conjugates with prototypical concanavalin A (Con A), voltammograms showed a decrease of the peak current. Such dendrimers showed a notable improvement of redox sensing abilities toward Con A when compared with mono- and divalent analogues, based on both the glycoside multivalent and ferrocene dendritic effects.