Rapid Fluorescence Screening Assay for Enrofloxacin and Tetracyclines in Chicken Muscle
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- 作者：Marilyn J. Schneider
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：December 29, 2004
- 年：2004
- 卷：52
- 期：26
- 页码：7809 - 7813
- 全文大小：75K
- 年卷期：v.52,no.26(December 29, 2004)
- ISSN：1520-5118
文摘
A simple, rapid fluorescence assay was developed for screening both enrofloxacin (ENRO) andtetracyclines in chicken muscle at the U.S. tolerance levels (300 ng/g and 2 
g/g, respectively).Screening for both classes of antibiotics is accomplished using one extraction, thus simplifying andexpediting the process. The method requires an initial extraction of chicken muscle with 1% aceticacid in acetonitrile, centrifugation, and analysis of the supernatant for ENRO fluorescence. Afteraddition of ammonium hydroxide, magnesium chloride, and methanol, followed by centrifugation andfiltration, the supernatant can be measured for tetracycline fluorescence. Chlortetracycline (CTC)was chosen as a representative tetracycline to demonstrate the method, as it displays intermediatesensitivity among the three tetracyclines approved in the U.S. Comparison of the fluorescence ofcontrol and tolerance-level-fortified samples of both ENRO and CTC shows no overlap. Setting athreshold as the average fortified fluorescence minus 3
allows for successful screening, as illustratedwith blind samples as controls or fortified with ENRO and/or CTC over a range of concentrations.This method can provide an alternative or supplemental approach to currently used microbial screeningassays.Keywords: Fluorescence; screening; enrofloxacin; fluoroquinolone; tetracyclines; chicken muscle
g/g, respectively).Screening for both classes of antibiotics is accomplished using one extraction, thus simplifying andexpediting the process. The method requires an initial extraction of chicken muscle with 1% aceticacid in acetonitrile, centrifugation, and analysis of the supernatant for ENRO fluorescence. Afteraddition of ammonium hydroxide, magnesium chloride, and methanol, followed by centrifugation andfiltration, the supernatant can be measured for tetracycline fluorescence. Chlortetracycline (CTC)was chosen as a representative tetracycline to demonstrate the method, as it displays intermediatesensitivity among the three tetracyclines approved in the U.S. Comparison of the fluorescence ofcontrol and tolerance-level-fortified samples of both ENRO and CTC shows no overlap. Setting athreshold as the average fortified fluorescence minus 3 allows for successful screening, as illustratedwith blind samples as controls or fortified with ENRO and/or CTC over a range of concentrations.This method can provide an alternative or supplemental approach to currently used microbial screeningassays.Keywords: Fluorescence; screening; enrofloxacin; fluoroquinolone; tetracyclines; chicken muscle